Differentially expressed genes between benign lymphoepithelial lesions of lacrimal gland and mucosa-associated lymphoid tissue lymphoma / 中华实验眼科杂志

Objective:To screen and analyze the differentially expressed genes between lacrimal gland benign lymphoepithelial lesions (LGBLEL) and mucosa-associated lymphoid tissue (MALT) lymphoma.Methods:A cross-sectional study was performed.Ten consecutive patients were included in Beijing Tongren Hospital Affiliated to Capital Medical University from January 2015 to November 2017, including five patients with LGBLEL and five patients with MALT lymphoma.Clinical data and peripheral blood sample were collected from each patient.DNA was extracted from peripheral blood.The whole-exome sequencing (WES) was employed for gene sequencing.The BWA software was used for the screen of differentially expressed gene; GATK software was used to detect genomic variation; ANNOVAR software was used to annotate and predict the effects of the variation; Varscan software was used to analyze single nucleotide polymorphisms (SNPs) and insertion-deletions (InDels), and ExomeCNV software was used to identify copy number variations (CNVs). The mutated hub gene with the maximal clique centrality was screened out by the analysis of protein interaction network and construction of functional module network.This study was approved by an Ethics Committee of Beijing Tongren Hospital Affiliated to Capital Medical University.Written informed consent was obtained from each patient prior to any medical examination.Results:There was 16.63 Gb sequencing data per sample on average.Synonymous mutation and missense mutation were the most common SNPs mutation types in the LGBLEL group and MALT lymphoma group, and no significant difference was found in gene mumber of synonymous mutation and missense mutation between the two groups.The number of terminating codon missing mutation genes in the LGBLEL group was more than that in the MALT lymphoma group ( P<0.05). The most common InDels types were frameshift mutation, non-frameshift insertion and non-frameshift deletion, and there was no significant difference in gene number of InDels between the LGBLEL group and MALT lymphoma group.The number of exon CNVs was few in both two groups and showed no significant influence in final result.Six differentially expressed hub genes were found, including IGFN1, TCP10, SLC45A4, BTBD7, PHGR1 and PIEZ02. Conclusions:IGFN1, TCP10, SLC45A4, BTBD7, PHGR1 and PIEZ02 genes may participate in the development of LGBLEL into MALT lymphoma.

The visual basis of the biological clock "sees" the light:intrinsically photosensitive retinal ganglion cells / 中华实验眼科杂志

Mammalian eyes mediate both image-forming and non-image-forming visual functions. Non-image-forming vision provides a measure of the ambient light for the purposes of synchronization of circadian clocks to light/dark cycles and regulation of pupil size,pineal melatonin production and other functions. Traditionally,people used to believe that the classical photoreceptors (rods and cones) regulate both image-forming and non-image-forming visual pathways. However,a small subset of retinal ganglion cells called intrinsically photosensitive RGCs ( ipRGCs) has been identified to be a third type of mammalian photoreceptor and determined to be photosensitive, recently. The discovery of ipRGCs has allowed for rapid progress in the past decade toward understanding the non-image-forming visual system,especially about how the circadian clock complete the synchronization with the light/dark cycle. The anatomical and developmental characteristics of ipRGCs, as well as its biological functions and regulation were reviewed in this paper.

Design of Rehabilitation Therapy Practice Base Using International Standards / 中国康复理论与实践

@#Objective To focus on how to bring the international teaching methods, assessment methods and indicators of the rehabilitation clinical practice on the basis of the local conditions.Methods By analyzing the problems found in the years of clinical teaching, and combining with research status home and abroad, the clinical teaching standards of WCPT were analyzed, and the design of establishing international standard rehabilitation practical base in local area was proposed. This paper discussed and shared our learning and suggestion in practice.Results This paper solved a series of issues of this clinical practical base, such as positioning, training methods and assess, and broke the traditional teaching mode of emphasizing pedagogical part.Conclusion It is very important to strengthen the skills training, improve the assessment system, and establish multiple assessment ways to clinical skills.

Research advance in application of stem cells in optic nerve regeneration / 眼科研究

Lots of blinding diseases are caused by retinal ganglion cells apoptosis, but there is no the effective and ideal therapy in clinic currently. Recent study showed that stem cells can be an alternative renewable source of retinal ganglion cells, and they may be potential to repair the visual function. These results provide a new model of optic nerve regeneration for the treatment of these blinding diseases. But, some problems in clinical applications are waiting for further solving. Applications of stem cells in optic nerve regeneration is reviewed in this paper.

The First Step of Lunching Biological Rocket Experiment in China——The Integral Results on Four Physiological Parameters of Dog / 航天医学与医学工程

Objective The effects of flight factors on dog and rats are observed to acumulate the elementary data and experiences for space biology research and development of life support systems.It will be the first basic step for manned space flight in future.Methods Female dogs,Xiao-Bao and Shan-Shan were on board of two Model T-7A(S2)rockets separately.Duration of flight was about 25 min and height about 70 km.Four physiological parameters:electrocardiogram,blood pressure,breath and skin temperature were recorded.Results Both of two dogs returned safely and healthy.The physiological parameters of Xiao-Bao were successfully recorded on the first rocket.On the second one,partial data of Shan-Shan were interfered by the strong vibration during the separation of rocket body and cone.The obvious changes of heart rate,ECG and blood pressure were recorded for Xio-Bao to be affected by the effects of hyper-gravity and microgravity factors.These changes were normal,temporal and reversible physiological stress responses.Conclusion The dog can tolerate the action of variable factors during the T-7A(S2)flight and no pathological after-effects were observed.